Molecular Determinants of pH Sensitivity of the Type IIa Na/Pi Cotransporter
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چکیده
منابع مشابه
Molecular determinants of pH sensitivity of the type IIa Na/P(i) cotransporter.
Type II Na/P(i) cotransporters play key roles in epithelial P(i) transport and thereby contribute to overall P(i) homeostasis. Renal proximal tubular brush border membrane expresses the IIa isoform, whereas the IIb isoform is preferentially expressed in small intestinal brush border membrane of mammals. IIa and IIb proteins are predicted to contain eight transmembrane domains with the N- and C-...
متن کاملSubstrate interactions in the human type IIa sodium-phosphate cotransporter (NaPi-IIa).
We have characterized the kinetics of substrate transport in the renal type IIa human sodium-phosphate cotransporter (NaPi-IIa). The transporter was expressed in Xenopus laevis oocytes, and steady-state and pre-steady-state currents and substrate uptakes were characterized by voltage-clamp and isotope flux. First, by measuring simultaneous uptake of a substrate (32Pi, 22Na) and charge in voltag...
متن کاملPhosphophloretin sensitivity of rabbit renal NaPi-IIa and NaPi-Ia.
The effect of phosphorylated phloretins on Na(+)-dependent phosphate uptake into rabbit renal brush-border membrane vesicles (BBMV) was examined. Na(+)-dependent phosphate uptake into isolated rabbit cortex BBMV was sensitive to 2'-phosphophloretin (2'-PP) and 2'-phospho-4',4,6'-trimethoxy phloretin (PTMP) in a dose-dependent and pH-dependent manner. PTMP inhibition of Na(+)-dependent phosphate...
متن کاملA dibasic motif involved in parathyroid hormone-induced down-regulation of the type IIa NaPi cotransporter.
Type II NaPi cotransporters are expressed in the apical membrane of P(i)-(re)absorbing epithelia: the type IIa in renal proximal tubule and the type IIb in small intestine. Parathyroid hormone (PTH) leads to a retrieval from the apical membrane of the type IIa NaPi cotransporter. The type IIa cotransporter is also expressed in opossum kidney (OK) cells, and its expression is under the control o...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 2000
ISSN: 0021-9258
DOI: 10.1074/jbc.275.9.6284